Determining the frequency of Epstein-Barr virus (EBV) in two inflammatory lesions of the periapical cyst and dental granuloma

Abstract

Background: One of the significant problems of Partial root canal therapy is periapical cyst and granuloma. Microorganisms contribute to the creation of periapical lesions, causing chronic illness and dental granuloma. This study aims to determine the effect of Epstein-Barr virus (EBV) on the exacerbation of the disease using the PCR technique. Methods: Paraffin tissue samples containing 25 periapical cysts, and 25 dental granulomas underwent DNA extraction. The extracted DNA quality was confirmed by the β-globin component replication using polymerase chain reaction (PCR). Moreover, the samples were evaluated using the nested-PCR technique in terms of the frequency of EBV using dedicated primers. The data analysis was performed using chi-squared, Fischer, and independent t-tests. Results: EBV was detected in 4 (1/25) of the periapical sample and 4 (1/25) of the 25 dental granuloma samples. Despite the above observations, there was no statistically significant difference in the frequency of EBV between the periapical cyst and dental granuloma samples. Conclusion: Based on the results of the study, EBV is likely to affect the pathogenesis or the growth of periapical lesions. Given that there is not a lot of research in this area and the results of the studies are conflicting, the findings attract more research. © 2019 Elsevier Inc.