Gene expression patterns of neurotrophin receptors during neuronal differentiation of human exfoliated deciduous teeth

Abstract

Objective: In the current study, we aimed to investigate the expression profile of TrkA, TrkB, TrkC, and p75NTR neurotrophin receptors because of their roles in the functional differentiation of human exfoliated deciduous teeth (SHED) cells into neural-like cells before and after differentiation of SHED cells into neural-like cells. Design: Total RNAs isolated from dental pulp tissue, SHED cells, and neural-like cells were reverse transcribed into complementary DNA. Neurotrophin receptor expression at mRNA and protein levels were compared in these three cell types by means of real-time PCR and western blot methods. Results: TrkA mRNA increased (713.6 +/- 251.5) significantly (p < 0.01) in neural-like cells difference from SHED and TrkB mRNA enhanced to 3618 times in these cells. The expression pattern of TrkC was very similar to the pattern of TrkA, and B. p75NTR mRNA increased 41.99 +/- 21.61 fold in neural-like cells and 9.805 +/- 4.06 fold in SHED cells. Almost the same pattern was observed for the expression of these receptors at the protein levels. Alterations with different grades and trends in neurotrophin receptors mRNA and protein expression levels were observed in these cells. Conclusion: Neurotrophin receptors are important in the existence and differentiation of SHED cells into neuron cells. Therefore, because of the neurogenic potential and accessibility of SHED cells, derived cells from SHED cells can be distinguished as an ideal source for tissue engineering.