Comparison of the Prevalence of Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) among Staphylococcus aureus Isolates in a Burn Unit with Non-Burning Units

Abstract

Background: Staphylococcus aureus (S. aureus) is one of the most important pathogens in bum infections colonized in the nose and increase the risk of infections. Methods: Overall, 85 S. aureus isolates were isolated from clinical and nasal hospitalized patients and health care workers (HCWs) in a bum unit and non-bum units in Isfahan from June 2016 and September 2016. Genes encoding penicillin-binding protein 2a (mecA) and adhesive surface proteins, including fibronectin-binding proteins (fnbA, fnbB), fibrinogen binding protein (fib), lamintn.-binding protein. (eno), collagen binding protein. (cna), elastin binding protein (ebps), intracellular adhesion op eron (icaA and icaD) were detected using PCR method. Results: The rate of methicilltn.-resistant S. aureus (MRSA) among bum and non-bum isolates were 62 (18/29) and 25 (14/56), respectively. The most prevalent MSCRAMMs genes in bum units were eno (86) and fib (66). The most common gene pattern in bum center was icaA+fib+ eno. The frequency of icaD, fib and ebpS was higher in clinical samples than nasal samples. No relation was found between the MSCRAMMs genes in the bum unit and non-bum units. Conclusion: The high prevalence of MRSA in burn center can be a new challenge for clinicians. The higher frequency of icaD, fib and e;,S in clinical isolates than nasal isolates may reflect the important role of these genes in colonization and pathogenesis of S. aureus.