Silica nano particles embedded in random and aligned PLGA/gelatin electrospun nano fibers improve growth and differentiation of human adipose-derived stem cells into anterior neuroectodermal cells

Abstract

Stem cell therapy is a promising approach involving the application of pluripotent cells for the treatment of a vast variety of diseases. There are several reports on designing and development of methods for suitable differentiation of stem cells to neural lineage. In this study, we investigated the potency of align and random poly(lacticco-glycolic acid) (PLGA) nano-fibers containing mesoporous silica nanoparticles (MSNPs) in differentiation of human adipose derived stem cells (hADSCs) into anterior neuroectodermal cells. The expression level of four anterior neuroectodermal markers including OTX2, SIX3, PAX6 and RAX was quantified for better understanding of the effect of scaffolds on cell differentiation. According to the obtained results, it seems that align PLGA/ MSNPs scaffolds are suitable for anterior neuroectodermal induction of hADSCs. Considering the absence of chemical agents for cell differentiation and with respect to the surprisingly high level of all four markers in the aligned fibers, using these scaffolds is introduced as a safe, cheap and powerful method for easy differentiation of hADSCs into anterior neuroectodermal cells.