New Cytotoxic Premyrsinane-Type Diterpenes from Euphorbia aleppica Against Breast Cancer Cells

(2022) New Cytotoxic Premyrsinane-Type Diterpenes from Euphorbia aleppica Against Breast Cancer Cells. Iranian Journal of Pharmaceutical Research. ISSN 17350328 (ISSN)

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Abstract

Euphorbia is used in traditional medicine to remove warts, possibly due to its cytotoxic or antiviral effects. This study investigated its phytochemistry and bioactive compounds. Euphorbia aleppica from the Euphorbiaceae family was collected from Kuhdasht, Lorestan, Iran. Plant material was dried and ground. Extraction was performed by maceration using a dichloromethane-acetone solvent. After removing fatty contents, fractionation was done by open column chromatography. Based on the initial H-NMR spec-tra, fractions containing diterpenoid compounds were identified. The Sephadex column and HPLC performed isolation. The HPLC was done with a regular YMC silica column using a hexane: Ethyl acetate (70: 30) solvent. The selected sub-fractions were identified by one and two-dimensional corelative NMR spectra. Accurate mass spectra confirmed the molecular formula of the obtained structures. Cytotoxicity was assessed using a standard MTT assay against breast cancer cells. The NMR and mass analysis identified compound 1 as a newly described and compound 2 as a pre-defined compound as 3, 7, 15β-triacetyl-5α-tigliate-13(17)-α-epoxy-14-oxopremyrsinane and 3, 7, 14, 15, 17-pentaacetyl-5-tigliate-13(17)-epoxypremyrsinane, respectively. Compound 1 showed moderate cytotoxicity, and compound 2 exhibited a potent cytotoxic effect dose-dependently against MCF-7 and MDA-MB 231 breast cancer cells, probably because of 14-O-acetyl and 17-O-acetylated hemiacetal groups. © 2022, Author(s).

Item Type: Article
Keywords: Breast Cancer Diterpene Euphorbia aleppica MCF-7 MDA-MB 231 Premyrsinane acetic acid ethyl ester dichloromethane diterpenoid sephadex silica gel solvent unclassified drug Article cancer cell carbon nuclear magnetic resonance cell proliferation assay cell viability assay column chromatography cytotoxicity electrospray mass spectrometry Euphorbia Euphorbiaceae female fractionation high performance liquid chromatography human human cell IC50 MCF-7 cell line MDA-MB-231 cell line MTT assay nuclear magnetic resonance phytochemistry proton nuclear magnetic resonance thin layer chromatography triple quadrupole mass spectrometry
Journal or Publication Title: Iranian Journal of Pharmaceutical Research
Journal Index: Scopus
Volume: 21
Number: 1
Identification Number: https://doi.org/10.5812/ijpr-127028
ISSN: 17350328 (ISSN)
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/16813

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