Time course of renal ischemia/reperfusion and distance organ: lung dysfunction in male and female rats

Abstract

Introduction: Renal ischemia/reperfusion injury (IRI) may influence distance organ such as lung. The severity of IRI induced-lung insult can be affected by gender and reperfusion time alteration. The aim of this study was to determine gender differences in renal IR induced-lung injury in different reperfusion time. Methods: Eighty male and female rats were assigned into 8 groups, 4 groups in each gender including: sham, renal ischemia for 45min by clamping renal vessels followed by 3, 24 or 48h reperfusion. Blood samples were obtained for measuring the serum level of blood urea nitrogen (BUN), creatinine (Cr), nitrite and malondialdehyde (MDA). The kidneys and lung tissues were removed and used for MDA and nitrite measurements and the histological changes evaluation. The lung water content was calculated. Results: In both genders, the rise in Cr and BUN reached the peak at 24h reperfusion. A significant increment was seen in female serum level of nitrite compared with males after 3h reperfusion. The renal MDA level of male increased during 3h reperfusion but not seen in females. The enhanced lung and renal tissues damages were depended to reperfusion time in both genders. The water content of lung was reduced in 3h of reperfusion groups. Conclusion: Sex effects and the time of reperfusion may be the important factors to consider clinical therapeutic of renal IRI as well as its impact on remote organs. © 2022, Iranian Society of Physiology and Pharmacology. All rights reserved.