Insights into the biochemical features and immunogenic epitopes of common bradyzoite markers of the ubiquitous Toxoplasma gondii

(2021) Insights into the biochemical features and immunogenic epitopes of common bradyzoite markers of the ubiquitous Toxoplasma gondii. INFECTION GENETICS AND EVOLUTION. ISSN 1567-1348 1567-7257 J9 - INFECT GENET EVOL

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Abstract

The widespread distribution of Toxoplasma gondii (T. gondii) infection and its harsh outcomes in pregnant women and immunocompromised patients lead researchers towards vaccination strategies. The present in silico investigation was done to reveal biophysical properties and immunogenic epitopes of six bradyzoite markers for rational vaccine design in future. For this purpose, different web servers were used to predict antigenicity, allergenicity, solubility, physicochemical properties, post-translational modification sites (PTMs), the presence of signal peptide and transmembrane domains. Moreover, the secondary and tertiary structures of the proteins were revealed followed by refinement and validation. Finally, NetCTL server was used to predict cytotoxic Tlymphocyte (CTL) epitopes, with subsequent immunogenicity analysis. Also, IEDB server was utilized to predict helper T-lymphocyte (HTL) epitopes, followed by IFN-gamma and IL-4 induction, antigenicity and population coverage analysis. As well, several linear antigenic B-cell epitopes were found, with good water solubility and without allergenicity. Totally, these proteins showed appropriate antigenicity, abundant PTMs as well as many CTL, HTL and B-cell epitopes, which could be directed for future vaccination studies in the context of multi-epitope vaccine design.

Item Type: Article
Keywords: Toxoplasma gondii Bradyzoite markers Immunoinformatics Immunogenic epitopes B-CELL EPITOPES IMMUNE-RESPONSES DNA VACCINATION INNATE IMMUNITY PREDICTION TACHYZOITES PROTEOMICS BIOLOGY HOST MICE
Journal or Publication Title: INFECTION GENETICS AND EVOLUTION
Journal Index: ISI
Volume: 95
Identification Number: https://doi.org/10.1016/j.meegid.2021.105037
ISSN: 1567-1348 1567-7257 J9 - INFECT GENET EVOL
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/17514

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