Evaluation of the effect of smoke of Anbar Nasara (Donkey dung) extract on staphilococcus aureus and bacilus subtilis

Abstract

Background: The use of medicinal smokes in treatment of many diseases, including infectious microbial diseases, has long been popular in Iran. This study aimed to compare the antimicrobial effects of smoke of Anbar Nasara (donkey dung) extract on Staphylococcus aureus and Bacillus subtilis with common antibiotics. Methods: In this experimental study, donkey dung smoke as medicine and antibiotics as a control group were considered. Standard strains of Staphylococcus aureus and Bacillus subtilis were cultured in suitable mediums. Anbar Nasara extract was prepared without use of solvent, in order to prevent the antibacterial effect of solvent on microbes. Dilution and disk diffusion test was performed 3 times for each of the bacteria. Findings: The mean of inhibition zone of Staphylococcus aureus by Anbar Nasara smoke was significantly greater than the inhibition zone induced by vancomycin; but there was no significant difference between the inhibition zone of Bacillus subtilis induced by Anbar Nasara smoke and ciprofloxacin. Least significant difference (LSD) post hoc test showed that the inhibition diameter of Staphylococcus aureus was significantly higher than the Bacillus subtilis. Bacillus subtilis induced higher minimum inhibitory concentration (MIC) values than Staphylococcus aureus; but, Bacillus subtilis induced higher minimum bactericidal concentration (MBC) values than Staphylococcus aureus. Conclusion: The smoke of Anbar Nasara extract has a significant inhibitory effect on Staphylococcus aureus growth; so it can be offered as a medicine. © 2017, Isfahan University of Medical Sciences(IUMS). All rights reserved.