Electrospun poly(hydroxybutyrate)/chitosan blend fibrous scaffolds for cartilage tissue engineering

Abstract

In this study, polyhydroxybutyrate (PHB) was blended with chitosan (CTS), and electrospun in order to produce more hydrophilic fibrous scaffolds with higher mass loss rates for cartilage tissue engineering application. First, the effects of diverse factors on the average and distribution of fiber's diameter of PHB scaffolds were systematically evaluated by experimental design. Then, PHB 9 wt solutions were blended with various ratios of CTS (5, 10, 15, and 20) using trifluoroacetic acid as a co-solvent, and electrospun. The addition of CTS could decrease both water droplet contact angle from approximate to 74 degrees to approximate to 67 degrees and tensile strength from, approximate to 87 MPa to approximate to 31 MPa. According to the results, the scaffolds containing 15 and 20 CTS were selected as optimized scaffolds for further investigations. Mass loss percentage of these scaffolds was directly proportional to the amount of CTS. Chondrocytes attached well to the surfaces of these scaffolds. The findings suggested that PHB/CTS blend fibrous scaffolds have tremendous potentials for further investigations for the intended application. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44171.