Ag-ZnO nanoparticles: synthesis, characterization, antibacterial activity on S. mutans, along with cytotoxic effect on U87 cell line

Abstract

In here, we introduced a rapid and green synthesis route to prepare pure zinc oxide (ZnO) and silver-doped zinc oxide (Ag-dop-ZnO) nanoparticles (NP) using Prosopis farcta extract. The physic-chemical property of the synthesized NPs was identified using PXRD, FESEM, FT-IR, UV-Vis, and EDX devices. According to the obtained outcomes of PXRD, UV-Vis, as well as EDX analysis, Ag was well doping into the ZnO structure. Moreover, spherical shape was observed with a mean size of 25-40 nm based on FESEM outcomes for the synthesized nanoparticles. The cytotoxic effect of the synthesized nanoparticles was assessed on glioblastoma (U87) cell line using an MTT test. The cytotoxic outcomes presented that the doped NPs have a more cytotoxic effect on the U87 cell line. The antibacterial activity of synthesized NP was studied against Streptococcus mutans bacterium through disk diffusion and microdilution method. Based on the outcomes of microdilution and disk diffusion assay and of the synthesized NP, Ag-dop-ZnO NP shows higher antibacterial activity against Streptococcus mutans than the pure NPs. Hence, the synthesized NPs can be suggested for disinfectant products and cancer treatment. © Nanomedicine Research Journal 2022. All rights reserved.