Facile, rapid and efficient isolation of circulating tumor cells using aptamer-targeted magnetic nanoparticles integrated with a microfluidic device

(2022) Facile, rapid and efficient isolation of circulating tumor cells using aptamer-targeted magnetic nanoparticles integrated with a microfluidic device. Rsc Advances. pp. 32834-32843.

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Abstract

Facile and sensitive detection and isolation of circulating tumor cells (CTCs) was achieved using the aptamer-targeted magnetic nanoparticles (Apt-MNPs) in conjugation with a microfluidic device. Apt-MNPs were developed by the covalent attachment of anti-MUC1 aptamer to the silica-coated magnetic nanoparticles via the glutaraldehyde linkers. Apt-MNPs displayed high stability and functionality after 6 months of storage at 4 degrees C. The specific microfluidic device consisting of mixing, sorting and separation modules was fabricated through conventional photo- and soft-lithography by using polydimethylsiloxane. The capture efficiency of Apt-MNPs was first studied in vitro on MCF-7 and MDA-MB-231 cancer cell lines in the bulk and microfluidic platforms. The cell capture yields of more than 91 were obtained at the optimum condition after 60 minutes of exposure to 50 mu g mL(-1) Apt-MNPs with 10 to 10(6) cancer cells in different media. CTCs were also isolated efficiently from the blood samples of breast cancer patients and successfully propagated in vitro. The isolated CTCs were further characterized using immunofluorescence staining. The overall results indicated the high potential of the present method for the detection and capture of CTCs.

Item Type: Article
Keywords: cancer-cells breast-cancer anti-muc1 aptamer capture muc1 immobilization enrichment separation functionalization optimization Chemistry
Page Range: pp. 32834-32843
Journal or Publication Title: Rsc Advances
Journal Index: ISI
Volume: 12
Number: 51
Identification Number: https://doi.org/10.1039/d2ra05930d
Depositing User: خانم ناهید ضیائی
URI: http://eprints.mui.ac.ir/id/eprint/24940

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