Sequence-identification of Candida species isolated from candidemia

Abstract

BACKGROUND: Candida species are the most prevalent cause of invasive fungal infections such as candidemia. Candidemia is a lethal fungal infection among immunocompromised patients worldwide. Main pathogen is Candida albicans but a global shift in epidemiology toward non-albicans species have reported. Species identification is imperative for good management of candidemia as a fatal infection. The aim of the study is to identify Candida spp. obtained from candidemia and determination of mortality rate among this population. MATERIALS AND METHODS: The study was performed during February 2014 to March 2015 in Tehran, Iran. Two-hundred and four blood cultures were evaluated for fungal bloodstream infection. Identification of isolates was carried out using phenotypic tests and polymerase chain reaction sequencing technique. RESULTS: Twenty-two out of 204 patients (10.8) had candidemia. Candida parapsilosis was the most prevalent species (45.4), followed by C. albicans (31.8) and Candida glabrata (22.7). Male to female sex ratio was 8/14. CONCLUSIONS: The emergence of resistant strains of Candida species should be considered by physicians to decrease the mortality of this fatal fungal infection by appropriate treatment.