Evaluating the effects of ultrasound waves on MCF-7 cells in the presence of Ag nanoparticles

Abstract

Background: Biological effects of ultrasound waves and their applications in the presence of nanoparticles are a rapidly growing research area. In recent years, ultrasonic therapy of cancer cells has been developed successfully. In this study, effects of ultrasound waves in the presence of Ag nanoparticles on the MCF-7 cells were evaluated. Methods: The MCF-7 cells were cultured. The cells were divided into 4 groups including: (1) control, (2) incubated with Ag nanoparticle, (3) treated using ultrasound alone and (4) treated using ultrasound at the presence of Ag nanoparticle. For groups of 2 and 4, nanoparticles were injected into the cells in 50, 100 and 200 μg/ml concentrations. Ultrasound irradiation at 2 W/cm2 intensity was performed on the cells for 3 min. Findings: Statistical comparison of the results showed that, there was a significant difference in the percent of cell viability between the US treated group at the presence of Ag and the control group (P < 0.05). On the basis of obtained results, the cell viability for the Ag incubated group at 200 μg/ml concentration was significantly different compared to the control group (P < 0.05). Conclusion: Results of this study revealed that ultrasound in the presence of Ag nanoparticles can be efficiently used for MCF-7 cells treatment. © 2016, Isfahan University of Medical Sciences(IUMS). All rights reserved.