Preparation of fibrin/poly vinyl alcohol electrospun nanofibers scaffold for tissue engineering applications

Abstract

Background: Nowadays, the biodegradable polymer nano-composites have particular importance in tissue engineering because of mechanical properties and good biocompatibility. The aim of this study was to design and evaluate nano-composite fibrin/polyvinyl alcohol (PVA) scaffold using electrospinning method and cell viability on it. Methods: Nano-composite scaffold fibrin/PVA were prepared by electrospinning method while 28.5 of the polymer was formed of fibrin. The porosity of the scaffolds was calculated via scanning electron microscopy by using “Matlab” software and porosity morphology, their distribution and size of the nanofibers. Water absorption test and contact angle measurement were performed. Also, human adipose-derived stem cells were used for cell viability evaluation on scaffolds. Findings: The mean diameter of electrospun fibrin/PVA scaffold was measured 500 nm. The average pore size and porosity of the prepared sample was 1.7 micrometers and 83.81, respectively. The average contact angle was 31.71 degrees and 24-hour average water absorption was measured 68.5. Evaluation test of the cell viability has a significant difference compared to control groups. Conclusion: The results of this study show that electrospun scaffolds fibrin/PVA can be used in cartilage and nerve tissue engineering. © 2016, Journal of Isfahan Medical School. All rights reserved.