The effect of ω-fatty acids on the expression of phospholipase A2 group 2A in human gastric cancer patients

(2016) The effect of ω-fatty acids on the expression of phospholipase A2 group 2A in human gastric cancer patients. Journal of Research in Medical Sciences. ISSN 17351995 (ISSN)

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Abstract

Background: Studies show that polyunsaturated fatty acids (PUFAs) may have an inhibitory role in carcinogenesis. It was previously shown that PLA2 group 2A (PLA2G2A) messenger RNA (mRNA) expression is associated with less frequent metastasis and longer survival in gastric adenocarcinoma. This study intends to investigate the effect of PUFAs on the expression of PLA2G2A in patients with gastric cancer. Materials and Methods: Thirty-four patients with gastric cancer (GC) were randomly divided into two groups. The first group received cisplatin medication. The second group received cisplatin medication and supplements of ω-fatty acids for three courses. The total RNA was extracted from the tissues and cDNA was synthesized. The gene expression of PLA2G2A was evaluated by the real-time polymerase chain reaction (PCR) method. To confirm the changes in gene expression, frozen section was utilized. The frozen tissue samples were sectioned and stained using the immunohistochemistry technique. Results: After chemotherapy and chemotherapy plus supplement, the relative mean of PLA2G2A gene expression increased 1.5 ± 0.5-fold and 7.4 ± 2.6-fold, respectively (P = 0.006). The relative mean of gene expression in patients who received cisplatin and ω-fatty acids supplement increased more significantly (7.5 ± 3.3-fold) than in patients who received only cisplatin (P = 0.016). Conclusion: It was found that PUFAs increased the gene and protein expression of PLA2G2A in gastric cancer. Concerning the fact that studies reveal protective function of PLA2G2A in gastric cancer, it is suggested that increased expression of PLA2G2A is helpful. Furthermore, PUFAs can be considered as a useful therapeutic supplement for patients with gastric cancer. © 2016 Journal of Research in Medical Sciences.

Item Type: Article
Keywords: Gastric cancer (GC) Phospholipase A2(PLA2) ω-fatty acids arachidonic acid borage oil cisplatin complementary DNA fish oil icosapentaenoic acid linoleic acid linolenic acid linseed oil omega 3 fatty acid omega 6 fatty acid phospholipase A2 group II polyunsaturated fatty acid messenger RNA adult Article cancer patient clinical article controlled study fatty acid analysis female gene expression human human tissue immunohistochemistry male protein expression real time polymerase chain reaction RNA isolation stomach cancer aged cancer chemotherapy computer assisted tomography endoscopy gene PLA2G2A gene RNA analysis stomach biopsy
Journal or Publication Title: Journal of Research in Medical Sciences
Journal Index: Scopus
Volume: 21
Number: 1
Identification Number: https://doi.org/10.4103/1735-1995.177358
ISSN: 17351995 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.mui.ac.ir/id/eprint/4335

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