Identification of Genomic Species of Acinetobacter Isolated from Burns of ICU Patients

Abstract

Background: The worldwide emergence of multi-drug resistant (MDR) bacteria in recent years has caused many problems for hospitals and patients, especially intensive care unit patients. Among these:clinically important MDR bacteria are Acinetobacter baumannii complex species (A. baumannii, Acinetobacter genomic species 3 and Acinetobacter genomic species 13TU) that cause a wide range of infections. Methods: The sequencing and bioinformatics analysis of a part of the Zone 1 of rpoB gene was performed for species identification of Acinetobacter isolates obtained from ICU patients with infected burns hospitalized in a hospital in Isfahan, Iran, over a 9-month period. Antibiotic Sensitivity of Acinetobacter isolates-was investigated using the disk diffusion method and different classes of antibiotics including amikacin, cefotaxime, ceftriaxone, ciprofloxacin, imipenem and piperacillin. Results: Acinetobacter spp. were isolated from 10 of 80 (12.5) investigated patients. All of the 10 Acinetobacter isolates were identified as Acinetobacter baumannii and multi-drug resistant according to antibiotic susceptibility teats. Conclusion: Of the Acinetobacter baumannii complex members, only A. baumannii species was identified among,the isolates obtained from patients with infected burns in an Isfahan hospital over a 9-month period.