AT1R A1166C variants in patients with type 2 diabetes mellitus and diabetic nephropathy

Abstract

BACKGROUND: There are inconsistent reports related to the role of angiotensin II type 1 receptor (AT1R) on the risk of type 2 diabetes mellitus (T2DM) and its renal complications. OBJECTIVES: To identify the association between AT1R A1166C variants with the risk of T2DM and also with diabetic nephropathy (DN). PATIENTS AND METHODS: In a case-control study, the AT1R A1166C polymorphism was detected in 135 T2DM patients with and without DN and in 98 healthy subjects from Western Iran. The genotypes of AT1R A1166C polymorphism were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. RESULTS: The frequencies of AT1R A1166C genotypes and alleles were not significantly difference between patients with and without DN and controls. The frequencies of rare allele of 1166 C were 10, 16.5, 15.9 and 15.3 in micro-, macro- and normo-albuminuric patients and in healthy individuals, respectively ( P > 0.05). The systolic blood pressure and serum creatinine level in DN patients were significantly higher in carriers of AT1R CC compared to carriers of AT1R AA genotype. In the presence of uncontrolled hyperglycemia (HbA1c > 7.5), there was a trend toward increased risk of macro-albuminuria in carriers of AC+CC genotype (OR=3.66, 95% CI: 0.81-16.58, P = 0.092). CONCLUSIONS: Our study indicated the absence of an association between AT1R A1166C polymorphism with the risk of T2DM and DN. It seems in carriers of AT1R C allele systolic blood pressure and serum creatinine level to be higher compared to the A allele carriers.