Detection of Legionella spp. by a nested-PCR assay in air samples of a wastewater treatment plant and downwind distances in Isfahan

(2015) Detection of Legionella spp. by a nested-PCR assay in air samples of a wastewater treatment plant and downwind distances in Isfahan. Advanced biomedical research. p. 48. ISSN 2277-9175 (Print) 2277-9175 (Linking)

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Abstract

BACKGROUND: Wastewater contains a variety of pathogens and bio -aerosols generated during the wastewater treatment process, which could be a potential health risk for exposed individuals. This study was carried out to detect Legionella spp. in the bio -aerosols generated from different processes of a wastewater treatment plant (WWTP) in Isfahan, Iran, and the downwind distances. MATERIALS AND METHODS: A total of 54 air samples were collected and analyzed for the presence of Legionella spp. by a nested- polymerase chain reaction (PCR) assay. A liquid impingement biosampler was used to capture bio -aerosols. The weather conditions were also recorded. RESULTS: Legionella were detected in 6 of the samples, including air samples above the aeration tank (1/9), belt filter press (1/9), and 250 m downwind (1/9). CONCLUSION: The result of this study revealed the presence of Legionella spp. in air samples of a WWTP and downwind distance, which consequently represent a potential health risk to the exposed individuals.

Item Type: Article
Keywords: Air Bio -aerosol Legionella spp. nested- PCR wastewater treatment plant
Page Range: p. 48
Journal or Publication Title: Advanced biomedical research
Journal Index: Pubmed
Volume: 4
Identification Number: https://doi.org/10.4103/2277-9175.151540
ISSN: 2277-9175 (Print) 2277-9175 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.mui.ac.ir/id/eprint/5843

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