Investigation the effect of the exposure time of docetaxel on MCF-7 cell line: An in-vitro assessment

Abstract

Background: Chemotherapy is one of the most applied methods in cancer treatment. Selection of the most effective cytostatic drug for cancer treatment is an important step in chemotherapy. Docetaxel is a drug that has especial effect when it combined with ionization radiation. The main effect of docetaxel is to block the cell cycle at the G2/M phase which has special sensitivity to the ionizing radiation. Different factors can impress the combination effect of their usage; one of them is exposure time of docetaxel with cells. In this study, we provided an experimental study about different exposure-time-induced effects of docetaxel on MCF-7 cells. Methods: We divided cells in three groups of control and 1 and 2 which were treated with different concentration of docetaxel for 5 or 24 hour, respectively. In-vitro cell viability tests were done to investigate their effect on MCF-7 cell line for 24, 48 and 72 hours after the experiment. Findings: The cytotoxicity was depended on the concentration of docetaxel. There was a significant decrease in cell viability at all concentration, especially in high concentrations for group 2 compared with group 1 which indicated time-and dose-dependency of the drug. Conclusion: Our study demonstrated that exposure time has an important effect on viability of the cells were treated with docetaxel. This phenomenon can affect treatment of cancer, especially in the field of combination therapy. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved.