Novel electrospun nanofibers of modified gelatin-tyrosine in cartilage tissue engineering

(2017) Novel electrospun nanofibers of modified gelatin-tyrosine in cartilage tissue engineering. Materials Science & Engineering C-Materials for Biological Applications. pp. 240-251. ISSN 0928-4931

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Abstract

In natural cartilage tissues, chondrocytes are linked to extracellular matrix (ECM) through cell-surface binding proteins. Surface modification of gelatin can provide a new generation of biopolymers and fibrous scaffolds with chemical, mechanical, and biological properties. In this study tyrosine protein and 1,2,3-triazole ring were utilized to functionalize gelatin without Cu catalyst. Their molecular structure was characterized by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy ((HNMR)-H-1). Chemical cross linkers such as glutaraldehyde (GA) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysulfosuccinimide (NHS) were used to electrospin the modified gelatin. The modification of gelatin and cross-linking effects were confirmed by scanning electron microscopy (SEMJ, contact angle measurement, and mechanical tests. MTT assay using chondrocyte cells showed cell viability of electrospun modified gelatin scaffolds. In vitro cell culture studies showed that electrospun engineered protein scaffolds would support the attachment and growth of cells. The results also showed that cross-linked nanofibers with EDC/NHS could be considered excellent matrices in cell adhesion and proliferation before electrospinning process and their potential substrate in tissue engineering applications, especially in the field of cartilage engineering. (C) 2016 Published by Elsevier B.V.

Item Type: Article
Keywords: nanofibers gelatin triazole tyrosine cartilage tissue engineering cross-linking cell-adhesion protein delivery click chemistry scaffolds hydrogels microspheres fabrication chitosan glutaraldehyde
Divisions: Biosensor research center
Page Range: pp. 240-251
Journal or Publication Title: Materials Science & Engineering C-Materials for Biological Applications
Journal Index: ISI
Volume: 71
Identification Number: https://doi.org/10.1016/j.msec.2016.10.003
ISSN: 0928-4931
Depositing User: مهندس مهدی شریفی
URI: http://eprints.mui.ac.ir/id/eprint/818

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