All Trans Retinoic Acid activates the RAR-beta and p14 tumor suppressor genes in OVCAR-3 cell line

Abstract

In this study, we investigated the effect of All Trans Retinoic Acid (ATRA) on the expression of the Retinoic Acid Receptor-beta(RAR-beta) and p14, two important tumor suppressor genes, in human ovarian cancer cells. OVCAR-3 cells were treated by ATRA and the mRNA levels of RAR-beta and p14 were measured by real time PCR. The percentage of apoptotic cells also evaluated by flow cytometry assay and cell viability determined by MTT method. Our real time results showed that the expression of RAR-beta and p14 gene in 48h treated groups was dramatically up-regulated by ATRA, while in 24 hours treated groups, there was no significant differences between the RAR-beta and p14 gene expression in comparison to the control group. The flow cytometry results showed that ATRA can significantly induce cell apoptosis at 48 hours. The cell viability in both 24 and 48 h treated groups were significantly lower than the control group. In order to these results we concluded that ATRA can up regulate the RAR-beta and p14 gene expression in OVCAR-3 ovarian cancer cell line.