Effect of Teucriumpolium on oxidative damages and sperm parameters in diabetic rat induced with streptozotocin

Abstract

Diabetes is one of the most prevalent metabolic diseases. It raises oxidative stress and thereby causes testicular damage. Utilization of antioxidants culminates in a reduction in oxidative stress and so brings about the testicular damage. In this study, we assessed the anti-oxidative potential of Teucrium polium extract on oxidative damages in the testes and sperm parameters of diabetic rats. In this experimental study, 32 male Wistar rats were segregated in 4 groups: Control, Diabetic and Diabetic treated with two doses of Teucrium poliumextract. An instillation of distilled water was performed in the control and diabetic groups. Also, treatment groups received T. poliumextract (50 and 100 mg/kg body weight) for 6 weeks. After treatment period all of animals were anesthetized, their blood sample were taken from Harte and measured the serum level of insulin and glucose thenthe testicles and epididymis wereremovedthenthe sperm parameters and oxidative stress markers were assessed. Treatment of diabetic rats with T. polium extract significantly reducedthe glucose plasma levels (p=0.001) and increased insulinlevels (p=0.001). Moreover, during diabetesan upsurge in the level of MDA and a decreasein the levels of SOD and CAT enzymes activity were observed in the testes. Administration ofT. polium extract (100 mg/kg BW) significantly rectified these parameters (p<0.05), but no significant differences were observed between 50 mg/kg BW of T. polium and diabetic group. In addition, the sperm parameters decreased in diabetic group, while the use of T. poliumsignificantly improved the above disorders in treatment groups (p<0.05). The results of this study confirm the antioxidant role of hydro-alcoholic extract of T. polium in amelioration the testicular oxidative damage caused by diabetes.